Съдържание:
  • Study of protein phosphorylation in intact cells
  • Analysis of signal transduction pathways using protein kinase inhibitors and activators
  • Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors
  • Analysis of signal transduction pathways using molecular biological manipulations
  • Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates
  • Phosphorylation site analysis by mass spectrometry
  • Assay and purification of protein (serine/threonine) phosphatases
  • Identification of substrates for protein-tyrosine phosphateases
  • Assay and purification of protein-serine/threonine kinases
  • Assay of protein kinases and phosphatases using specific peptide substrates
  • Cloning and expression of cDNAs encoding protein kinase subunits
  • Interaction cloning of protein kinase partners
  • Identifying protein kinase substrates by expression screening with solid-phase phosphorylation
  • Analysis of protein kinase interactions using the two-hybrid method
  • Analysis of protein kinase interactions using biomolecular interaction analysis
  • Analysis of protein kinase specificity using oriented peptide libraries
  • Crystallization of protein kinases and phosphatases.