Arabidopsis : a practical approach /
| Други автори: | Wilson, Zoe A. |
|---|---|
| Формат: | Електронна книга |
| Език: | English |
| Публикувано: |
Oxford ; New York :
Oxford University Press,
2000.
|
| Серия: |
Practical approach series ;
223. |
| Предмети: | |
| Онлайн достъп: |
http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98516 |
| Подобни документи: |
Print version::
Arabidopsis. |
Съдържание:
- 1.
- Growth, maintenance, and use of Arabidopsis genetic resources /
- Mary Anderson, Fiona Wilson
- 1
- 1.
- What is Arabidopsis?
- 1
- 2.
- What makes Arabidopsis such an attractive experimental model?
- 3
- 3.
- Arabidopsis genetic resource centres
- 4
- Arabidopsis genetic resources
- 4
- Accessing Arabidopsis resources
- 5
- 4.
- Mutants of Arabidopsis
- 5
- Single gene mutation lines
- 5
- Resources for the identification/investigation of novel genes
- 8
- Mapping tools
- 10
- 5.
- Considerations of available resources for identifying novel genes
- 11
- Forward genetics
- 12
- Reverse genetics
- 14
- 6.
- Growing Arabidopsis
- 15
- How to maintain clean growth conditions
- 15
- Growing Arabidopsis in the glasshouse
- 16
- Chemical control of pests and diseases
- 19
- 7.
- Seed storage
- 21
- 8.
- Growing Arabidopsis with specific growth requirements
- 23
- 9.
- Sterile culture of Arabidopsis
- 24
- 2.
- Preservation and handling of stock centre clones /
- Randy Scholl, Keith Davis, Doreen Ware
- 29
- 2.
- Missions of a plant DNA resource centre
- 29
- 3.
- Preservation of stocks
- 30
- Plasmids with small DNA inserts
- 30
- Cosmids
- 32
- Phage and phage libraries
- 33
- Yeast artificial chromosome (YAC) libraries
- 33
- Pools of YAC library cells for PCR screening
- 37
- Distribution of YAC libraries arrayed on nylon filters
- 38
- Other large-insert libraries
- 38
- Yeast expression analysis
- 'two-hybrid' libraries and complementation testing
- 42
- 4.
- Verification of stock identity and purity
- 42
- 5.
- Pooled DNA from T-DNA lines for PCR screening
- 44
- 6.
- Organization of stock information
- 48
- Collecting, maintaining, and disseminating stock data
- 48
- Organizing and distributing patron data
- 48
- 7.
- Future
- 48
- 3.
- Genetic mapping using recombinant inbred lines /
- Clare Lister, Mary Anderson, Caroline Dean
- 51
- 2.
- Preparation and digestion of A. thaliana genomic DNA
- 54
- Preparation of genomic DNA
- 56
- Identifying an RFLP
- 60
- Southern blotting and hybridization
- 61
- 3.
- Polymorphic markers
- 62
- RFLP markers
- 62
- PCR-based markers
- 66
- Phenotypic and biochemical markers
- 68
- 4.
- Calculating map positions
- 69
- Mapping programs
- 69
- NASC mapping service
- 70
- 5.
- Integration of a mutation into a molecular map
- 72
- 4.
- Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis /
- Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson
- 77
- 1.
- Generation of mutants and their importance for developmental biology
- 77
- 2.
- Mapping and segregation analysis
- 78
- Mapping of mutations
- 78
- Influence of environment on phenotype
- 82
- 3.
- Microscopy
- 82
- Fresh material characterization
- 82
- Fixed material characterization
- 84
- 4.
- Analysis of plant gene expression
- 89
- RNA isolation
- 89
- Northern analysis
- 91
- In situ hybridizations
- 92
- 5.
- Classical and molecular cytogenetics of Arabidopsis /
- G.H. Jones, J.S. Heslop-Harrison
- 105
- 2.
- Mitotic chromosome analysis by light microscopy
- 108
- 3.
- In situ hybridization to mitotic chromosome preparations
- 112
- Photography of in situ hybridizations
- 117
- 4.
- Meiotic chromosome analysis by light microscopy
- 117
- 5.
- Meiotic chromosome analysis by electron microscopy
- 121
- 6.
- Tissue culture, transformation, and transient gene expression in Arabidopsis /
- Keith Lindsey, Wenbin Wei
- 125
- 2.
- Stable transformation by Agrobacterium tumefaciens
- 128
- 3.
- Transient gene expression in Arabidopsis protoplasts
- 131
- Reporter gene enzyme assays
- 134
- 7.
- Transposon and T-DNA mutagenesis /
- Mark G.M. Aarts, Csaba Koncz, Andy Pereira
- 143
- 2.
- Transposon tagging
- 143
- Endogenous transposable elements
- 143
- Transposon tagging systems in Arabidopsis
- 144
- Which system to use?
- 149
- Genetic and molecular analysis of a putatively transposon tagged mutant
- 151
- Further applications of transposon tagging
- 156
- 3.2
- T-DNA tagging
- 158
- Use of T-DNA as insertional mutagen
- 158
- Random tagging
- 158
- Available populations of T-DNA transformants
- 159
- Promoter/enhancer trapping
- 160
- Analysis of T-DNA mutants and cloning a tagged gene
- 161
- Further applications of T-DNA tagging
- 166
- 8.
- Map-based cloning in Arabidopsis /
- Joanna Putterill, George Coupland
- 171
- 2.
- Locating the mutation of interest relative to DNA markers
- 172
- Determining an approximate map position
- 172
- Identifying a short genetic interval containing the mutation as a prelude to isolating the gene
- 175
- 3.
- Placing the gene on the physical map
- 177
- Chromosome landing
- 177
- Chromosome walking with YAC clones
- 178
- 4.
- Identification of the gene
- 189
- Location of the gene by molecular complementation
- 189
- Determining the structure of the gene
- 194
- 5.
- Perspectives
- 194
- 9.
- Physical mapping: YACs, BACs, cosmids, and nucleotide sequences /
- Ian Bancroft
- 199
- 2.
- Genome mapping with YAC clones
- 199
- 3.
- Genome mapping with BAC and P1 clones
- 202
- Communal resources
- 202
- Construction of BAC libraries
- 208
- Genome mapping with BACs
- 211
- 4.
- High resolution mapping with cosmids
- 216
- Approaches to mapping with cosmids
- 216
- Construction of cosmid libraries
- 216
- 5.
- Nucleotide sequences
- the ultimate mapping tool
- 221
- EST sequencing project
- 221
- Genome sequencing project
- 221
- Sequence-based mapping
- 222
- 10.
- Web-based bioinformatic tools for Arabidopsis researchers /
- Seung Y. Rhee, David J. Flanders
- 225
- What is bioinformatics
- 225
- Sources of Arabidopsis bioinformatic data
- 226
- 2.
- Basic tools for the Internet
- 227
- Web basics
- 227
- Getting onto the Web
- 228
- Using your browser
- 229
- Browser tips and errors
- 232
- Privacy issues
- 235
- 3.
- Scenarios of bioinformatic use in Arabidopsis research
- 235
- 4.
- Gene information resources
- 237
- General gene information
- 237
- Arabidopsis gene information
- 237
- Plant gene information
- 238
- Genetic maps
- 239
- Physical maps
- 240
- 6.
- Sequencing
- 242
- Arabidopsis genome initiative (AGI)
- 243
- Annotation of sequences by AGI
- 243
- Caveats in annotation
- 244
- Sequence contigs from AtDB
- 245
- 7.
- Sequence analysis tools
- 245
- BLAST
- 246
- FASTA
- 250
- EST databases
- 252
- Gene identification programs
- 253
- Gene family analyses
- 255
- Motif analyses
- 255
- Protein structures
- 258
- Comprehensive sequence analysis tools
- 259
- 8.
- Current issues and future directions in bioinformatics
- 260
- Some important bioinformatic issues
- 260
- Bioinformatic tools currently under development for Arabidopsis research.