Инженерство на функционални нуклеинови киселини и приложението им в областите на молекулярната генетика и синтетичната биология : Автореферат /
This dissertation is related with the recently emerging field in biology – Synthetic biology, and the application of its methods for the creation of new antibacterial agents. Utilizing the different The synthetic biology seeks and produces new ways to develop and use artificially derived molecules t...
| Основен автор: | Трайковска, Мартина 1990- |
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| Други автори: | Trajkovska, Martina 1990-, Пенчовски, Робърт Димитров (науч. ръководител) |
| Формат: | Книга |
| Език: | Bulgarian |
| Публикувано: |
София,
2017.
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| Предмети: | |
| Онлайн достъп: |
Пълен запис |
| Резюме: |
This dissertation is related with the recently emerging field in biology – Synthetic biology, and the application of its methods for the creation of new antibacterial agents. Utilizing the different The synthetic biology seeks and produces new ways to develop and use artificially derived molecules to perform important regulatory functions in the cell. These synthetic molecules bind with different molecule targets in the cell, that can be used to control the gene expression of genes involved in important metabolic pathways. The use of antisense oligonucleotides (ASOs) for the creation of new antibacterial agents that are effective against pathogenic bacteria that are resistant to the existing antibiotics. Riboswitches are functional, non-coding RNA domains with complex secondary and tertiary structure that are located in the 5'-untranslated region (UTR) of the bacterial mRNAs. These RNA domains control the expression of genes that have an important function for the cellular metabolism. The main two elements of the riboswitches are the effector domain and the expression platform. The use of ASO technology enables the use of bacterial riboswitches as targets for the detection of new antibacterial agents. The basis of this technology is the use of ASOs which hybridize with bacterial-specific mRNAs responsible for the synthesis of vital proteins. ASOs themselves contain modifications that make them more resistant to cellular nuclease enzymes. The experiments described in this Ph.D. thesis convincingly demonstrate the inhibition of the bacterial growth of three pathogenic bacteria in humans: Listeria monocytogenes, Staphylococcus aureus and Escherichia coli and the non-pathogenic bacterium Bacillus subtilis. My research on riboswitches, both on bioinformatics and laboratory, indicate that they are suitable targets for the creation of new antibacterial agents. This gives hope in the fight against antibacterial resistance in pathogenic bacteria that cause severe diseases in humans, many of which may have lethal consequences. In this sense, the submitted dissertation has not only a purely academic value in creating new method for proving the molecular function of bacterial riboswitches And the consequence of their inhibition in vivo, but there have also significant potential applications in the pharmaceutical industry to create new antibacterial agents. |
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| Описание на библ. документ: |
Кор. опис. |
| Физически характеристики: |
74 с. : с цв. ил. ; 21 см. |
| Библиография: |
Библиогр. с. 69-74 |