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Techniques in protein chemistry VIII /

Praise for the Series: "The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team."--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-...

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Автор-организации: Protein Society. Meeting
Други автори: Marshak, Daniel R.
Формат: Електронна книга
Език: English
Публикувано: San Diego ; London : Academic Press, ℗♭1997.
Предмети:
Proteins > Analysis > Congresses.
Peptides > Analysis > Congresses.
Nucleotide sequence > Congresses.
SCIENCE > Chemistry > Organic.
Nucleotide sequence.
Peptides > Analysis.
Proteins > Analysis.
Electronic books.
Conference papers and proceedings.
Онлайн достъп: http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=207165
Подобни документи: Print version:: Techniques in protein chemistry VIII.
Съдържание:
  • Foreword.
  • Preface.
  • Acknowledgments.
  • Primary Structural Analysis: Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection:
  • Enhancement of Concentration Limits of Detection in Capillary Electrophoresis: Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization.
  • Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS).
  • Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis.
  • Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques.
  • Comparison of the High Sensitivity and Standard Versions of Applied Biosystems Procise <V>TM</V> 494 N-Terminal Protein Sequencers Using Various Sequencing Supports.
  • Evaluation of ABRF-96SEQ: A Sequence Assignment Exercise.
  • Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol.
  • A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing.
  • Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study.
  • Physical and Chemical Analysis:
  • Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors: Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI Detectors.
  • Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection.
  • A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides.
  • Sample Preparation Using Synthetic Membrances for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry.
  • Use of LC/MS Peptide Mapping for Characteriation of Isoforms in <V>15</V>N-Labeled Recombinant Human Leptin.
  • Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests.
  • Detecting and Identifying Active Compounds from a Combinatorial Library Using Iasys and Electrospray Mass Spectrometry.
  • Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization.
  • Development of a Method for Analysis of Free Amino Acids from Physiological Samples Using a 420A ABI/PE Amino Acid Analyzer.
  • Quantitation and Identification of Proteins by Amino Acid Analysis: ABRF-96AAA Collaborative Trial
  • Chemical Modification:
  • Nonaqueous Chemical Modification of Lyophilized Proteins
  • Reaction of HIV-1 NC p7 Zinc Fingers with Electrophilic Reagents.
  • The Identification and Isolation of Reactive Thiols in Ricin A-Chain and Blocked Ricin Using 2-(4'-Maleimidylanilino)naphthalene-6-sulfonic Acid.
  • Inactivation of the Human Cytomegalovirus Protease by Diisopropylfluorophosphate.
  • Studies on the Status of Arginine Residues in Phospholipase A<->2 from Naja naja atra (Taiwan cobra) Snake Venom.
  • Selective Reduction of the Intermolecular Disulfide Bridge in Human Glial Cell Lined-Derived Neurotrophic Factor Using Tris-(2-Carboxyethyl)Phosphine.
  • Effects of Surface Hydrophobicity on the Structural Properties of Insulin.
  • The Effects of in Vitro Methionine Oxidation on the Bioactivity and Structure of Human Keratinocyte Growth Factor.
  • Posttranslational and Other Modifications:
  • Effects of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation.
  • Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling.
  • Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry.
  • Positive Identification of Glycosylation Sites in Proteins and Peptides Using a Modified Beckman LF 3600 N-Terminal Protein Sequencer.
  • Deamidation and Isoaspartate Formation during in Vitro Aging of a Recombinant Hepatitis E Vaccine Candidate.
  • The Isolation and Characterization of Active Site Peptides in Lysyl Oxidase.
  • Complement Activation in EDTA Blood/Plasma Samples May Be Caused by Coagulation Proteases.
  • Disulfide-Linked Human Stem Cell Factor Dimer: Method of Identification and Molecular Comparison to the Noncovalent Dimer.
  • Autocatalytic Reduction of a Humanized Antibody.
  • Interactions of Protein with Ligands:
  • Oxygen and Ascorbate Mediated Modification of a Recombinant Hemoglobin.
  • Metal Activation and Regulation of E. coli Rnase H. Crystal Structure of Avian Sarcoma Virus Integrase with Bound Essential Cations.
  • Multidimensional NMR Studies of an Exchangeable Apolipoprotein and Its Interactions with Lipids.
  • NMR Methods for Analysis of CRALBP Retinoid Binding.
  • Novel Method for Measuring the Binding Properties of the Site-Directed Mutants of the Proteins that Bind Hydrophobic Ligands: Application to Cellular Retinoic Acid Binding Proteins.
  • A Strategy for Predicting the Ligand Binding Competence of Recombinant Orphan Nuclear Receptors Using Biophysical Characterization.
  • Protein-Protein Interactions:
  • Detection of Intra-Cellular Protein-Protein Intractions: Penicillin Interactive Proteins and Morphogene Proteins.
  • Use of Synthetic Peptides in Mapping the Binding Sites for hsp70 in a Mitochondrial Protein.
  • Interfacing Biomolecular Interaction Analysis with Mass Spectrometry and the Use of Bioreactive Mass Spectrometer Probe Tips in Protein Characterization.
  • Transition-State Theory and Secondary Forces in Antigen-Antibody Complexes.
  • Thermodynamic Investigation of Enzyme and Inhibitor Interactions with High Affinity.
  • Development and Characterization of a Fab Fragment as a Surrogate for the IL-1 Receptor.
  • Macromolecular Assemblies:
  • Topogology of Membrane Proteins in Native Membranes Using Matrix-Assisted Laser Desorption Ionization/Mass Spectrometry.
  • Role of D-Ser<V>46</V> in the P-type Calcium Channel Blocker, w-Agatoxin-TK.
  • Involvement of Basic Amphiphilic a-Helical Domain in the Reversible Membrane Interaction of Amphitropic Proteins: Structural Studies by Mass Spectrometry, Circular Dichroism, and Nuclear Magnetic Resonance.
  • One-Dimensional Diffusion of a Protein along a Single-Stranded Nucleic Acid.
  • Metal-Dependent Structure and Self Association of the RAG1 Zinc-Binding Domain.
  • Localizing Flexibility within the Target Site of DNA-Bending Proteins.
  • Assembly of the Multifunctional EcoKI DNA Restriction Enzyme in Vitro.
  • Three Dimensional Structure:
  • Strategies for NMR Assignment and Global Fold Determinations Using Perdeuterated Proteins.
  • <V>1</V>H-NMR Evidence for Two Buried ASN Side-Chains in the c-MYC-MAX Heterodimeric a-Helical Coiled-Coil.
  • NMR Confirms the Presence of the Amino-Terminal Helix of Group II Phospholipase A<->2 in Solution.
  • The Crystallographic Analysis of Glycosylation-Inhibiting Factor
  • Structure of the D30N Active Site Mutant of FIV Proteinase Complexed with a Statine-Based Inhibitor.
  • A Homology-Based Model of Juvenile Hormone Esterase from the Crop Pest, Heliothis virescens.
  • Analysis of Linkers of Regular Secondary Structures in Proteins.
  • Structural and Functiona Roles of Tyrosine-50 of Yeast Guanylate Kinase.
  • Dynamics and Folding:
  • Flexibility of Serine Protease in Nonaqueous Solvent.
  • Internal Dynamics of Human Ubiquitin Revealed by <V>13</V>C-Relaxation Studies of Randomly Fractionally Labeled Protein.
  • Detection of Protein Unfolding and Fluctuations by Native State Hydrogen Exchange.
  • Laser Temperature Jump for the Study of Early Events in Protein Folding.
  • Biophysical and Structural Analysis of Human Acidic Fibroblast Growth Factor.
  • A Thermodynamic Analysis Discriminating Loop Backbone.
  • The Equilibrium Ensemble of Conformational States in Staphylococcal Nuclease.
  • An Evaluation of Protein Secondary Structure Prediction Algorithms.
  • Biological and Chemical Design:
  • Designing Water Soluble b-Sheet Peptides with Compact Structure.
  • Engineering Secondary Structure to Invert Coenzyme Specificity in Isopropylmalate Dehydrogenase.
  • A Method for Determining Domain Binding Sites in Proteins with Swapped Domains: Implications for bA3- and bB2- Crystallins.
  • Complete Mutagenesis of the Gene Encoding TEM-1 b-Lactamase.
  • Characterization of Truncated Kirsten-Ras Purified from Baculovirus Infected Insect Cells Indicates Heterogeneity due to N-terminal Processing and Nucleotide Dissociation.
  • Isolation and Characterization of Multiple-Methionine Mutants of T4 Lysozyme with Simplified Cores.
  • Synthesis of Alzheimer's (1-42) Ab-Amyloid Peptide with Preformed Fmoc-Aminoacyl Fluorides.
  • Analysis of Racemization during "Standard" Solid Phase Peptide Synthesis: A Multicenter Study.
  • Index.

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