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Techniques in protein chemistry VI /

TECHNIQUES IN PROTEIN CHEMISTRY VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and p...

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Автор-организации: Protein Society.
Други автори: Crabb, John W.
Формат: Електронна книга
Език: English
Публикувано: San Diego : Academic Press, ℗♭1995.
Предмети:
Онлайн достъп: http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=240756
Подобни документи: Print version:: Techniques in protein chemistry VI.
Съдържание:
  • Foreword.
  • Preface.
  • Acknowledgements.
  • Mass Spectrometry of Peptides and Proteins:
  • The Use of a Volatile N-Terminal Degradation Reagent for Rapid, High-Sensitivity Sequence Analysis of Peptides by Generation of Sequences Ladders, M. Bartlet-Jones, W.A. Jeffery, H.F. Hansen, and D.J.C. Pappin.
  • Investigation of Polyethylene Membranes as Potential Sample Supports for Linking SDS-PAGE with MALDI-TOF MS for the Mass Measurement of Proteins, J.A. Blackledge and A.J. Alexander.
  • Comparison of ESI-MS, LSIMS, and MALDI-TOF-MS for the Primary Structure Analysis of a Monoclonal Antibody, L. Cano, K.M. Swiderek, and J.E. Shively.
  • MS Based Scanning Methodologies Applied to Conus Venom, A.G. Craig, W.H. Fischer, J.E. Rivier, J.M. McIntosh, and W.R. Gray.
  • Direct Coupling of an Automated 2-Dimensional Microcolumn Affinity Chromatography-Capillary HPLC System with Mass Spectrometry for Biomolecule Analysis, D.B Kassell, T.G. Consler, M. Shalaby, P. Sekhri, N. Gordon, and T. Nadler.
  • Edman Degradation and MALDI Sequencing Enables N- and C-Terminal Sequence Analysis of Peptides, R. Kellner, G. Talbo, T. Houthaeve, and M. Mann.
  • Identification of the Amino Terminal Peptide of N-Terminally Blocked Proteins by Differential Deutero-Acetylation Using LC/MS Techniques, C.D. Thulin and K.A. Walsh.
  • Analysis of Posttranslational Processing Events:
  • HPAEC-PAD Analysis of Monoclonal Antibody Glycosylation, J. Rohrer, J. Thayer, N. Avdalovic, and M. Weitzhandler.
  • Carbohydrate Structure Characterization of Two Soluble Forms of a Ligand for the ECK Receptor Tyrosine Kinase, C.L. Clogston, P.L. Derby, R. Toso, J.D. Skrine, M. Zhang, V. Parker, G.M. Fox, T.D. Bartley, and H.S. Lu.
  • Characterization of Individual N- and O-Linked Glycosylation Sites Using Edman DegradationA. A. Gooley, N.H. Packer, A. Pisano, J.W. Redmond, K.L. Williams, A. Jones, M. Loughnan, and P.F. Alewood.
  • The Unexpected Presence of Hydroxylysine in Noncollagenous Proteins, M.S. Molony, S.-L. Wu, L.K. Keyt, and R.J. Harris.
  • Isolation of Escherichia coli Synthesized Recombinant Proteins that Contain (e>-N-Acetyllysine, B.N. Violand, M.R. Schlittler, C.Q. Lawson, J.F. Kane, N.R. Siegel, C.E. Smith, and K.L. Duffin.
  • LC-MS Methods for Selective Detection of Posttranslational Modifications in Proteins: Glycosylation, Phosphorylation, Sulfation, and Acylation, M.F Bean, R.S. Annan, M.E. Hemling, M. Mentzer, M.J. Huddleston, and S.A. Carr.
  • Identification of Phosphorylation Sites by Edman DegradationJ. D. Shannon and J.W. Fox.
  • Determination of the Disulfide Bonds of Human Macrophage Chemoattractant Protein-1 Using a Gas Phase Sequencer, R. Seetharam, J.I. Corman, and S.M. Kamerkar.
  • Protein Sequencing and Amino Acid Analysis:
  • Enzymatic Digestion of PVDF-Bound Proteins in the Presence of Glucopyranoside Detergents: Applicability to Mass Spectrometry, J. Fernandez, F. Gharahdaghi, and S.M. Mische.
  • In-Gel Digestion of SDS PAGE-Separated Proteins: Observations from Internal Sequencing of 25 Proteins, K.R. Williams and K.L. Stone.
  • Peptide Mapping at the 1 m> Level: In-Gel vs. PVDF Digestion Techniques, L.A. Merewether, C.L. Clogston, S.D. Patterson, and H.S. Lu.
  • Enzymatic Digestion of Proteins in Zinc Chloride and Ponceau S Stained Gels, S. Zhou and A. Admon.
  • Direct Collection Onto Zitex and PVDF for Edman Sequencing: Elimination of Polybrene, W.A. Burkhart, M.B. Moyer, W.M. Bodnar, A.M. Everson, V.G. Valladares, and J.M. Bailey.
  • Minimizing N-to-O Shift in Edman Sequencing, W.H. Vensel and G.E. Tarr.
  • The Hydrolysis Process and the Quality of Amino Acid Analysis: ABRF-94AAA Collaborative Trial, K. U˜, Yu˜ksel, T.T. Andersen, I. Apostol, J.W. Fox, R.J. Paxton, and D.J. Strydom.
  • A New Reagent for Cleaving at Cystine Residues, C. Mitchell, L. Hinman, L. Miller, and P.C. Andrews.
  • Protein Sequence Analysis Using Microbore PTH Separations, M.F. Rohde, C. Clogston, L.A. Merewether, P. Derby, and K.D. Nugent.
  • Assignment of Cysteine and Tryptophan Residues during Protein Sequencing: Results of ABRF-94 SEQ, J. Gambee, P.C. Andrews, K. DeJongh, G. Grant, B. Merrill, S. Mische, and J. Rush.
  • Automated C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System, C.G. Miller, D.H. Hawke, J. Tso, and S. Early.
  • Applications Using an Alkylation Method for Carboxy-Terminal Protein Sequencing, M.L. Bozzini, J. Zhao, P.-M. Yuan, D. Ciolek, Y.-C. Pan, J. Horton, D.R. Marshak, and V.L. Boyd.
  • C-Terminal Sequence Analysis of Polypeptides Containing C-Terminal Proline, J.M. Bailey, O. Tu, G. Issai, and J.E. Shively.
  • Peptide and Protein Separations and Other Methods:
  • High Sensitivity Detection of Tryptic Digests Using Derivatization and Fluorescence Detection, S.A. Cohen, I. Mechnikov, and P. Young.
  • Reagents for Rapid Reduction of Disulfide Bonds in Proteins, R. Singh and G.M. Whitesides.
  • Strategies for the Removal of Ionic and Nonionic Detergents from Protein and Peptide Mixtures for On- and Off-Line Liquid Chromatography Mass Spectrometry (LCMS), K.M. Swiderek, M.L. Klein, S.A. Hefta, and J.E. Shively.
  • Online Preparation of Complex Biological Samples prior to Analysis by HPLC, LC/MS, and /or Protein Sequencing, K. Stoney and K. Nugent.
  • Methods for the Purification and Characterization of Calcium-Binding Proteins from Retina, A.S. Polans, K. Palczewski, W.A. Gorczyca, and J.W. Crabb.
  • Evidence for the Presence of a>-Bungarotoxin in Venom-Derived k>-Bungarotoxin, J.J. Fiordalisi and G.A. Grant.
  • Progress in the Development of Solvent and Chromatography Systems Appropriate for Bitopic Membrane Proteins, S.-J. Kil, L.M. Oleksa, G.C. Landis, and C.R. Sanders, II.
  • Rapid Separation of Proteins and Peptides Using Conventional Silica-Based Supports: Identification of 2-D Gel Proteins following In-Gel Proteolysis, R.L. Moritz, J. Eddes, H. Ji, G.E. Reid, and R.J. Simpson.
  • Mutagenesis and Protein Design:
  • Studying a>-Helix and b>-Sheet Formation in Small Proteins, C.K. Smith, M. Munson, and L. Regan.
  • Circular Permutation of RNase T1 through PCR Based Site-Directed Mutagenesis, J.M. Kuo, L.S. Mullins, J.B. Garrett, and F.M. Raushel.
  • E. coli-Expressed Human Neurotrophin-3 Characterization of a C-Terminal Extended Product, J.O. Hui, S.-Y. Meng, V. Katta, L. Tsai, M.F. Rohde, and M. Haniu.
  • Spectral Enhancement of Recombinant Proteins with Tryptophan Analogs: The Soluble Domain of Human Tissue Factor, C.A. Hasselbacher, R. Rusinova, E. Rusinova, and J.B.A. Ross.
  • Utilization of Partial Reactions, Side Reactions, and Chemical Rescue to Analyze Site-Directed Mutants of Ribulose 1,5-Bisphosphate (RuBP) Carboxylase/Oxygenase (Rubisco), M.R. Harpel, E.H. Serpersu, and F.C. Hartman.
  • Probing the Roles of Conserved Histidine Residues in b>-Galactosidase (E. coli) Using Site Directed Mutagenesis and Transition State Analog Inhibition, N.J. Roth, K.Y.N. Wong, and R.E. Huber.
  • Analysis of Protein Interactions:
  • Rapid in Vitro Assembly of Class I Major Histocompatibility Complex, N.J. Papadopoulos, J.C. Sacchettini, S.G. Nathenson, and R.H. Angeletti.
  • Peptide Models of bZIP Proteins: Quantitative Analysis of DNA Affinity and Specificity, S.J. Metallo and A. Schepartz.
  • Applying Affinity Coelectrophoresis to the Study of Nonspecific, DNA Binding Peptides, M.L. Nedved and G.R. Moe.
  • Investigating Calmodulin-Target Sequence Interactions Using Mutant Proteins and Synthetic Target Peptides, W.A. Findlay, S.R. Martin, and P.M. Bayley.
  • Interactions of Bacterial Cell-Surface Proteins with Antibodies: A Versatile Set of Protein-Protein Interactions, G.C.K. Roberts, L.-Y. Lian, I.L. Barsukov, J.P. Derrick, K. Kato, and Y. Arata.
  • Studies of Cytokine-Cytokine Receptor Interactions: Influence of Ligand Dimerization, L.D. Ward, G.J. Howlett, R.L. Moritz, A. Hammacher, K. Yasukawa, and R.J. Simpson.
  • New High Sensitivity Sedimentation Methods: Application to the Analysis of the Assembly of Bacteriophage P22, W.F. Stafford, III, S. Liu, and P.E. Prevelige, Jr.
  • Protein Conformation and Folding:
  • Cyanogen as a Conformational Probe, R.A. Day, A. Hignite, and W.E. Gooden.
  • Evaluation of Interactions between Residues in a a>-Helices by Exhaustive Conformational Search, T.P. Creamer, R. Srinivasan, and G.D. Rose.
  • Design, Synthesis, and Characterization of a Water-Soluble b>-Sheet Peptide, D.S. Wishart, L.H. Kondejewski, P.D. Semchuk, C.M. Kay, R.S. Hodges, and B.D. Sykes.
  • Automated Analysis of Protein Folding, R.A. Smith, J. Henkin, and T.F. Holzman.
  • HSP70 Protein Complexes: Their Characterization by Size-Exclusion HPLC, D.R. Palleros, L. Shi, K. Reid, and A. Fink.
  • Methods for Collecting and Analyzing Attenuated Total Reflectance FTIR Spectra of Proteins in Solution, K.A. Oberg and A.L. Fink.
  • NMR Analysis of Peptides and Proteins:
  • <sup>19</sup>F NMR Studies of Fluorinated Sugars Binding to the Glucose and Galactose Receptor, L.A. Luck.
  • Heteronuclear Gradient-Enhanced NMR for the Study of 20-30 kDa Proteins: Application to Human Carbonic Anhydrase II, R.A. Venters and L.D. Spicer.
  • Toward the Solution Structure of Large (>30 kDa) Proteins and Macromolecular Complexes, C.H. Arrowsmith, W. Lee, M. Revington, T. Yamazaki, and L.E. Kay.
  • Solution Structures of Horse Ferro- and Ferricytochrome c Using 2D and 3D <sup>1</sup>H NMR and Restrained Simulated Annealing, P.X. Qi, E.J. Fuentes, R.A. Beckman, D.L. Di Stefano, and A.J. Wand.
  • NMR Relaxation Methods to Study Ligand-Receptor Interactions, D.W. Hoyt, J.-J. Wang, and B.D. Sykes.
  • Peptide Synthesis:
  • Application of 2-Chlorotrityl Resin: Simultaneous Synthesis of Peptides Which Differ in the C-Termini, A.L. Hong, T.T. Le, and T. Phan.
  • Correlation of Cleavage Techniques with Side-Reactions following Solid-Phase Peptide Synthesis, G.B. Fields, R.H. Angeletti, L.F. Bonewald, W.T. Moore, A.J. Smith, J.T. Stults, and L.C. Williams.
  • Protein Synthesis on a Solid Support Using Fragment Condensation, S. Brandtner and C. Griesinger.
  • Characterization of a Side Reaction Using Stepwise Detection in Peptide Synthesis with Fmoc Chemistry, Y. Yang, W.V. Sweeney, S. Tho˜rnqvist, K. Schneider, B.T. Chait, and J.P. Tam.
  • Erratum:
  • High Sensitivity Peptide Sequence Analysis Using in Situ Proteolysis on High Retention PVDF Membranes and a Biphasic Reaction Column Sequencer, S. Best, D.F. Reim, J. Mozdzanowski, and D.W. Speicher.
  • Index.